RNA LOC DROS · Identification of novel factors involved in RNA localization in Drosophila
6РП — Действия „Мария Кюри“
- Период
- 2006-10-01 → 2008-09-30
- Финансиране от ЕС
- 158 479 €
- Участници
- 1
- Схема
- EIF
Линиите свързват координатора с партньорите.
Накратко на български
Механизмите за транспортиране на РНК молекули се проучват в нервната система на плодовата мушка. Разбирането на този процес помага да се разберат образуването на тумори и създаването на спомени.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - RNA LOC DROS (Identification of novel factors involved in RNA localisation in Drosophila)
Unraveling RNA localisation in the fruitfly RNA molecules are the intermediates between DNA and proteins and are crucial for determining the activity and functions off all cells. Many RNAs are directed to specific sites inside cells. Two important functions for this intracellular RNA localisation are: 1. Asymmetric cell division, important for example in stem cell divisions, which in turn is implied in tumour formation and 2. Localised protein synthesis, important for example in memory formation. In many cases it is still an open question which genes are important for the process of RNA localisation. In this project the mechanisms of RNA transport and localisation were studied in the developing nervous system of the fruitfly, Drosophila. We have used a library of transgenic fly lines that express double stranded RNA of genes that are possibly involved in RNA localisation, resulting in a partially loss of function of these genes. Subsequently the effect on RNA localisation in de developing nervous system of the fruitfly was monitored in several ways. The results indicate that this approach is feasible and we can indeed disturb RNA localisation using this method, potentially allowing the identification of genes involved in this process. This project has resulted in a usable method to identify genes involved in RNA localisation.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Sub-cellular localisation of RNA has emerged as a key mechanism through which cells become polarized. The localisation of transcripts is an extremely efficient way to target gene products to subcellular compartments or to specific regions of a cell or embryo, making it an important posttranscriptional level of gene regulation. The aim of this project is to perform an RNA-interference (RNAi) screen to identify novel components required for intracellular mRNA transport in Drosophila. A wide range of Drosophila cell-lines will be characterized to select those with asymmetric morphology well suited to visualise localised transcripts. Initially, I will screen for dsRNAs that disrupt RNA localisation as assayed by sensitive fluorescence in situ hybridisation in fixed cells. First, I will examine transcripts that are known to localise in other systems, for example b-actin transcripts that are present at the leading edge of migrating fibroblasts and neurites, and later, identify possible target transcripts expressed i n the selected cells using Drosophila cDNA microarrays. Once a reliable assay has been set up I will begin by inactivating genes already implicated in RNA localisation or microtubule-based transport, and screen more widely when high-throughput methods have been established. Genes that affect localisation without grossly disrupting cytoskeleton organisation will be analysed further genetically and biochemically to examine effects on RNA localisation and development. This RNAi screen will likely lead to the identification of a large number of genes involved in different pathways of RNA localisation and/or individual steps of these pathways. These will include genes already known to be involved in these pathways, but also new candidates. Since the molecular motors involved in RNA localisation are the same motors responsible for transport of other cargos, such as lipid vesicles, the project could contribute to a more integral view on intracellular transport.
Оригинален текст от CORDIS (на английски).
Участници
- CANCER RESEARCH UK · LONDONКоординаторОбединеното кралство
Връзки
Данни: CORDIS, © Европейски съюз