NEURONAL IDENTITY · Regulation of odorant receptor choice
6РП — Действия „Мария Кюри“
- Период
- 2007-05-31 → 2008-05-30
- Финансиране от ЕС
- 40 000 €
- Участници
- 1
- Схема
- ERG
Линиите свързват координатора с партньорите.
Накратко на български
Генетичният код, който определя кои рецептори за миризми развиват невроните при плодовата муха, се анализира чрез премахване на конкретни гени. Това помага да се разбере как се създават различните видове неврони и как се формира обонятелната система.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - NEURONAL IDENTITY (Regulation of odorant receptor choice)
Can a Fruit fly give us answer how an olfactory system is created? Yes, our research point in that direction. Although flies use their antenna to detect odours and we our nose the principal organisation is the same: all neurons that respond to one and the same odour express the same odorant receptor and are converging their axons to one point in the brain thus creating an odorant map. The largest difference is that humans have about 500 different Odorant receptors and thus groups of olfactory neurons where as a fruit fly only has 52, a number that is small enough to be grasped and overviewed. The restricted size is important if one wants to understand how each group of neurons are generated, how can one create different neuronal types? To address this issue we have combined the small olfactory system with a novel genetic technology in Fruit fly, transgenic RNAi. This technology makes it possible to remove the function of any gene anywhere in the fly. In this way we have found 7 regulatory genes so called transcription factors which have been shown to be necessary to create olfactory neurons. Furthermore different combinations of factors seem to decide which group of olfactory neurons that the neuron will belong to. In other words each neuron in the antenna might be determined by a regulatory code of factors.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Within most olfactory systems, each neuron chooses to express only one specific odorant receptor out of a vast number of receptor genes. Understanding this complex process will lead to an increased knowledge not only on how the olfactory system is generated, but also provide a deeper insight into how cellular diversity is established in the human brain.The main objectives of this proposal are to genetically and molecularly address:- How is odorant receptor choice regulated at a transcriptional level?- What are the factors, DNA-sites and regulatory processes involved?The strategies for answering these questions are:- To identify molecules that specify odorant receptor choice, a work which we have initiated using a novel transgenic RNAi screen in Drosophila. The initial screen has identified 212 candidate genes that effect odorant receptor expression.- To perform an extensive phenotypic characterization followed by a more detailed analysis of a few selected genes, with the aim of further addressing specifically the molecular genetic interactions between the identified regulators.- To explore how conserved these novel findings might be.To this end, I plan to conduct parallel studies in vertebrate systems, using my previous acquired expertise in the mouse olfactory system. The proposed project will be beneficial at multiple levels:- It will link multiple aspects of development that have been unconnected in the drosophila olfactory community.- It will reveal novel genes and generate novel tools for addressing questions in other fields: epigenetics, cell signalling, stem cell research etc.- It will strengthen both the olfactory and cell fate research fields in Europe.- It will increase the scientific collaboration within Europe.
Оригинален текст от CORDIS (на английски).
Участници
- LINKÖPINGS UNIVERSITET · LINKÖPINGКоординаторШвеция
Връзки
Данни: CORDIS, © Европейски съюз