MARCYV · Marine cyanobacterial viruses: diversity and dynamics
6РП — Действия „Мария Кюри“
- Период
- 2007-10-01 → 2009-09-30
- Финансиране от ЕС
- 80 000 €
- Участници
- 1
- Схема
- IRG
Линиите свързват координатора с партньорите.
Накратко на български
Морските цианобактериални вируси в Червено море се анализират чрез техните гени и количество. Това помага да се разбере как се променя разнообразието на тези вируси според сезона и вида на бактерията, която заразяват.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - MARCYV (Marine cyanobacterial viruses: diversity and dynamics)
We have uncovered a vast diversity in the existing podovirus populations in the Red Sea using PCR and sequencing of 3 genes - the DNA polymerase gene, the major capsid protein gene and the photosynthesis gene psbA. These podoviruses can be divided into two major clusters with a number of other more minor groupings apparent. We have for the first time quantified podoviruses and myoviruses infecting marine cyanobacteria. We found that samples from the Red Sea in winter and summer harbour different numbers of these two major virus families infecting marine cyanobacteria and that their relative abundance differs with the host used for enumeration.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Viruses are extremely abundant in the marine environment and are thought to profoundly influence ecosystem functioning. As host-specific agents of mortality they influence the population dynamics, diversity and evolution of their hosts. However, we are presently unable to assess the abundance or dynamics of discrete viral populations.Furthermore, estimates of viral-induced mortality are rudimentary at best, due to the lack of appropriate tools to quantify infected host cells. I propose to investigate the diversity of viruses that infect the ecologically important marine cyanobacteria, Synechococcus and Prochlorococcus, as well as assess their impact on host mortality. I will focus on the ubiquitous podoviruses for which little information is presently available. The diversity of cyanobacterial podoviruses will be determined by sequencing the DNA polymerase gene from podovirus isolates.We will use these findings to develop primers for the specific identification of cyanobacterial podoviruses and will employ a novel molecular approach, solid-phase polymerase chain reaction, for their quantification. The abundance and population dynamics of these podoviruses will then be determined over spatial and temporal scales in the Red Sea and will be compared to host population and total virus dynamics.Furthermore, this method will be used to amplify viral DNA from single cyanobacterial cells to investigate the extent of podovirus infection. The results from this research will significantly enhance our understanding of the influence of podoviruses on cyanobacterial mortality, population dynamics and community composition, and thus their impact on primary production and recycling of matter in the oceans.I bring to this project and Europe, unique expertise acquired in the US on physiological, genomic and evolutionary aspects of cyanobacteria-virus interactions. EC funding of this project will advance my progress towards a permanent position at the Technion.
Оригинален текст от CORDIS (на английски).
Участници
- TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY · HAIFAКоординаторИзраел
Връзки
Данни: CORDIS, © Европейски съюз