LISA · And there was light, silver and DNA
6РП — Действия „Мария Кюри“
- Период
- 2006-12-01 → 2010-11-30
- Финансиране от ЕС
- 452 864 €
- Участници
- 1
- Схема
- TOK
Линиите свързват координатора с партньорите.
Накратко на български
Бактериалната ДНК от клинични проби се анализира чрез светлинна спектроскопия, усилена със сребърни наночастици. Този метод помага за по-точното разпознаване на патогени и подобрява процесите по изолиране на протеини и антитела.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Activity Report Summary - LISA (and there was Light, Silver and dnA)
In the LISA project the idea is to characterise bacterial DNA from clinical samples using silver nanoparticle enhanced light spectroscopy (called surface enhanced resonance Raman spectroscopy). LISA stands for "and there was Light Silver and dnA". Three Marie Curie Fellows have worked on the project. Fellow 1 - Andrew Derome has worked on a 3-plex SERRS assay after PCR from real clinical samples using new biotin-avidin clean technology. Fellow 2 - Ron Gill developed a simplified assay based on the DNA-induced aggregation of CTAB-coated silver nanoparticles. Also Ron Gill has researched and optimised conditions to get enhancement of fluorescence rather than Raman signal. Using such conditions fluorescence enhancement observed was comparable or even better than the best published results for surface enhanced fluorescence. The MC fellow has adapted the synthesis of silver colloids for small volumes (1ml) in glass tubes using a thermomixer (usually used in biology for doing heating reactions in eppendorf tubes). In that way it is much easier to do a large number of synthesis in parallel with different reactants concentrations. Fellow 3 - Gwenola Sabatte transferred knowledge in SERRS active anti-body conjugates for enrichment and elution methods for enrichment applications. She investigated silver and gold coated magnetic particles for magnetic beads immunoassays and also worked on optimisation of enrichment via elution for magnetic beads immunoassays. The final work she performed on the development of a protein enrichment assay.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Sepsis is a systemic response to an infection of the bloodstream by bacteria, which causes organ failure and death in severe cases. Annually, about 1.5 Million people worldwide suffer from sepsis and approximately 1400 people die daily from this disease. Early diagnosis and appropriate treatment can greatly improve survival rates. Currently, infectious diseases are diagnosed by studying vital signs (e.g. fever, rapid heart rate). Moreover, blood cultures are grown to detect the presence of bacteria. In general, to get results from blood culturing takes three days and a non-optimised therapy by a broad spectrum, a cocktail" of antibiotics is started before culturing results arrive. To clearly improve the treatment of patients, rapid diagnostic clinical tests are required. Sensitive technology is envisioned to reduce the analysis time from several days to a few hours. At Philips Research we have started studies on bacterial DNA detection, based on surface-enhanced resonant Raman spectroscopy (SERRS).SERRS enables sensitive and selective detection of molecules adsorbed at a metal surface. The scattered light consists of molecule-specific bands, which renders discrimination of multiple analytes possible. DNA of infectious bacteria is detected through the measurement of SERRS labels adsorbed at aggregated silver colloids. Advantages of SERRS are: high sensitivity (pico-molar) and the unique potential to simultaneously measure mixtures of DNA labels.To bring the SERRS method to such a level that it can be used as clinical tool, quite some effort is required and knowledge at Philips needs to be build up, especially in the following three areas:1. molecular biology and biochemistry,2. colloid and surface chemistry and3. bio-informatics, respectively.This proposal applies for 3 ToK Fellows, 24 months each, for our molecular diagnostics SERRS project in the upper three areas."
Оригинален текст от CORDIS (на английски).
Участници
- PHILIPS ELECTRONICS NEDERLAND B.V. · EINDHOVENКоординаторНидерландия
Връзки
Данни: CORDIS, © Европейски съюз