HIPENZO · Enzymatic synthesis of organic molecules in alternative solvents
7РП — „Хора“ (Действия „Мария Кюри“)
- Период
- 2008-07-01 → 2010-06-30
- Финансиране от ЕС
- 169 958 €
- Участници
- 1
- Схема
- MC-IEF
Линиите свързват координатора с партньорите.
Накратко на български
Ензимите се тестват в алтернативни разтворители, за да се синтезират органични молекули, като например полиестери. Това помага за създаването на по-екологични технологии за производство на лекарства и химикали, като се намали използването на вредни летливи разтворители.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Enzymatic synthesis of organic molecules in alternative solvents
A growing awareness of the need for greener, more sustainable technologies has focused attention on the use of atom efficient catalytic methodologies for the manufacture of fine chemicals and pharmaceuticals. The use of alternative reaction solvents such as supercritical fluids or fluorous biphasic systems that are readily recycled, such as fluorous solvents and scCO2 has therefore become the focus of significant research that could circumvent the problems associated with many of the traditional volatile organic solvents. Thus novel and effective methods, enzyme recovery and recycling are receiving increasing attention. New approaches tackling these issues were objectives of this project. Enzyme solubilisation in fluorous solvents and scCO2 by Hydrophobic Ion Pairing with perfluorinated surfactants was investigated. Various commercially available lipases capable of catalysing esterification and transesterification reactions were screened to select a lipase that could be efficiently ion paired with fluorinated surfactants. The complexation efficiencies of 60-70 % were achieved with several lipases giving 9-15 mg/ml [enzyme] in fluorous solvent. Two hydrophobically ion paired lipases Candida cylindracea and Pseudomonas cepacia demonstrated reasonable catalytic activity and good enantioselectivity towards the kinetic resolution of rac-1- phenylethanol in a perfluoromethylcyclohexane/hexane biphasic system. When HIP-lipases were employed for the ring-opening polymerisation of various lactones in fluorous biphasic system lipases Rhizopus arrhizus and Pseudomonas fluorescens were active and yielded polyesters. Further HIP-lipases were tested for the polycondensation polymerisation of polyesters. The ability to solubilise lipases in fluorinated solvents provided an opportunity to perform homogenous polymerisation of highly fluorinated monomers. Several hydroxyl-terminated fluorinated diols and fluorinated acids were examined in order to generate fluorinated polyesters with unique physical and chemical properties.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
The growing awareness of the pressing need for greener, more sustainable technologies has focused attention on the use of atom efficient catalytic methodologies for the manufacture of fine chemicals and pharmaceuticals. The use of alternative reaction solvents such as supercritical fluids or fluorous biphasic systems that are readily recycled has therefore become the focus of significant research that could circumvent the problems associated with many of the traditional volatile organic solvents. Thus novel and effective methods for reactor design, enzyme recovery and recycling are receiving increasing attention. New approaches to tackling these issues form the main objectives of this Proposal. The use of immobilized enzymes in the form of Cross-Linked Enzyme Aggregates (CLEAs) in supercritical carbon dioxide (scCO2) or fluorous reaction media including the use of ‘combi-CLEAs’, two enzymes that catalyse complementary reactions for one-pot two-step conversions in both batch and continuous reactions forms one major task. The use of hydrophobic-ion pairing of enzymes with perfluorinated surfactants to solubilise them in scCO2 and fluorous solvents and thus allow homogeneous catalysis to occur is the focus of the second task. The oxidases catalyzing the mild and clean formation of H2O2 from dioxygen will be investigated. This H2O2 will then be used in epoxidation and Baeyer-Villiger chemistry, either on the product of the oxidase reaction or a second substrate. Enzyme catalyzed polymerization reactions in scCO2 and fluorous solvents will also be investigated including the formation of perfluorinated polymers that may have unique physical and chemical properties. This fellowship will bring together strong research background of Dr. I. Lapenaite with excellent training opportunities at the University of Nottingham. This team aims to deliver significant breakthroughs in the development of new environmentally sustainable processing of organic materials.
Оригинален текст от CORDIS (на английски).
Участници
- THE UNIVERSITY OF NOTTINGHAM · NottinghamКоординаторОбединеното кралство
Връзки
Данни: CORDIS, © Европейски съюз