PERIOPAIN · Determination of the role of P. gingivalis and P. intermedia proteases in mono- and synergistic mixed microbial infections in a mouse model of periodontal disease
7РП — „Хора“ (Действия „Мария Кюри“)
- Период
- 2008-10-01 → 2012-09-30
- Финансиране от ЕС
- 100 000 €
- Участници
- 1
- Схема
- MC-IRG
Линиите свързват координатора с партньорите.
Накратко на български
Бактериите P. gingivalis и P. intermedia и техните ензими се анализират чрез миша модели, за да се види как предизвикват загуба на костна тъкан. Това помага да се разбере как микробите разрушават тъканите и как организмът реагира на инфекцията.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Determination of the role of P. gingivalis and P. intermedia proteases in mono- and synergistic mixed microbial infections in a mouse model of periodontal disease
Project context and objectives These anaerobic microorganisms are well equipped with many different virulence factors such as proteolytic enzymes, which we have been studying in vivo during the two last years of the following project. To determine the role of P. gingivalis and P. intermedia proteases in microbial infections, two different mouse models of periodontal disease were established in our lab. Bone loss model was used to test the role of P. gingivalis and P. intermedia proteases in mono- and synergistic mixed microbial infections. Both types of bacteria were able to induce bone loss in Balb/c mice separately. However, we didnt find any difference when the mixed microbial infection was performed. We focused than to test the importance of interpain in P.intermedia on bone loss model. We found that, the infection, the generation of Ab, and the bone loss was stronger when infection was performed with wild type P. intermedia. Moreover, we developed the mouse chamber model, which gave us the possibility to investigate the role of the host response in the bacterial infection. The subcutaneous chamber model has been described before as a model for the host tissue destruction that occurs in periodontitis. Using the subcutaneous chamber model, we were able to characterise the inflammatory mediator profile at the sites of infection by a periodontal pathogen, P. gingivalis and to compare the different strains of deficient bacteria. We found that peptidylarginine deiminase (PAD), which catalyses the deimination of peptidylarginine residues of various peptides is an important virulence factor of P. gingivalis. To reveal if P. g. infection might influence collagen induced arthritis (CIA) in DBA/1 mice, chamber model was employed. The results of our in vivo and in vitro investigation reveal that P. g. is able to enhance CIA in DBA/1 mice, and PPAD is an important virulence factor of this bacterium.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Periodontitis is a chronic inflammatory disease of the supporting structures of the teeth caused by a polymicrobial infection and, despite the general improvement of oral health status and advances in treatment; it continues to afflict a large percentage of adults worldwide. If morbidity caused by tooth loss due to advanced periodontitis was not enough, mounting evidence suggests a causative link between periodontal and cardiovascular diseases. It is now generally accepted that a relatively small consortium of anaerobic Gram-negative bacteria (P. gingivalis, T. denticola and T. forsythia) described as the “red complex” is strongly associated with the pathological changes in the periodontium. Each of these bacteria is well equipped with virulence factors among which, proteolytic enzymes are the best studied. In the case of P. gingivalis, gingipains were shown to significantly contribute to bacterial virulence in vivo. Proteolytic activity produced by P. intermedia may also add to the destructive potential of the red complex consortium since the presence of this bacterium strongly correlates with the presence of P. gingivalis and T. forsythia in the subgingival communities. We hypothesize that different proteases affect host defense system at different non-redundant sites and these concerted multiple assaults should lead to a significantly greater effect than the additive action of individual proteases. Therefore, we postulate that this kind of interaction occurs in the periodontal pocket and may be experimentally verified using specific mice models of periodontal disease using monomicrobial and synergistic mixed microbial infections with a combination of P. gingivalis and P. intermedia wild-type and protease-deficient mutants. Our proposed hypothesis of combined proteolytic periodontopathogen activity may have significant potential importance for the treatment and prevention of periodontal disease in the future.
Оригинален текст от CORDIS (на английски).
Участници
- Jagiellonian University in Krakow · KRAKOWКоординаторПолша
Връзки
Данни: CORDIS, © Европейски съюз