'RIBOSOME MM' · A Synthetic Molecular Machine Capable of Complex Task Performance: Processive Sequence-Selective Synthesis
7РП — „Хора“ (Действия „Мария Кюри“)
- Период
- 2009-03-01 → 2011-02-28
- Финансиране от ЕС
- 172 435 €
- Участници
- 1
- Схема
- MC-IEF
Линиите свързват координатора с партньорите.
Накратко на български
Синтетична молекулярна машина, базирана на ротаксани, се разработва за създаване на пептидни вериги според определена последователност. Успехът ѝ би създал опростен модел на рибозомата, което е най-сложната задача, изпълнявана досега от изцяло изкуствена система.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
A synthetic molecular machine capable of complex task performance: processive sequence-selective synthesis
The goal of this project is to transpose the information inscribed onto a molecular tape or thread into a product in a sequence-specific manner with the help of a molecular machine, specifically to develop a rotaxane-based molecular machine capable of sequence specific peptide synthesis. The project involved the development of methodology for efficient rotaxane assembly without strong intercomponent interactions within the product; design and construction of a track with appropriate blocking groups; model studies to develop transamidation chemistry; and finally, the assembly, characterisation and operation of the molecular machine. A reliable synthesis for each one of the building blocks of the rotaxane-based molecular machine was established, as well as an efficient and convergent method to bring them together. The construction of rotaxanes with one and two amino acid fragments has been optimised. Model studies have been completed and conditions for the operation of the molecular machine have been successfully established for simple test systems involving one amino acid residue. The achievement of synthesising and operating such a complex molecular machine, which could be regarded as a simplified molecular ribosome model, would be by far the most complex task ever performed by a purely synthetic system.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
The aim of this proposal is to synthesize and demonstrate the successful operation of one of the first synthetic molecular machines capable of performing a complex task at the molecular level. The target is to mimic translation, the process through which protein is synthesized on the mRNA template in the ribosome. To achieve this ambitious task (in a very basic form) through the action of a wholly artificial molecular machine system we propose to utilize a [2]rotaxane in which the macrocycle component acts as both a catalyst and a molecular transporter, abstracting bulky aromatic amino acid substituents from a sequence-specific ‘thread’ and transporting them in turn to the next amino acid fragment before mediating the formation of a new amide bond between them. The design is such that the macrocycle is forced to approach each amino acid in sequence, and is unable to pass until the cycle is complete, imparting sequential integrity to the oligopeptide synthesis. The mechanically interlocked nature of the rotaxane ensures processivity during the machine’s operation. Although biology uses threaded molecular architectures to transfer chemical information during sequence-specific oligomer and polymer assembly (proteins, oligo- and polysaccharides, DNA and RNA), such effects are unprecedented in artificial systems. We hope that our synthetic studies will provide a breakthrough in the development of artificial molecular machines that can perform real tasks at the molecular level including, ultimately, the synthesis of unnatural sequence-specific oligomers and polymers that are not accessible by other routes.
Оригинален текст от CORDIS (на английски).
Участници
- THE UNIVERSITY OF EDINBURGH · EdinburghКоординаторОбединеното кралство
Връзки
Данни: CORDIS, © Европейски съюз