CHROMATIN MOTILITY · An innovative system to impose altered features on a defined chromosome region and investigate its movement by fluorescence microscopy in the nucleus of a cell
7РП — „Хора“ (Действия „Мария Кюри“)
- Период
- 2009-05-01 → 2011-04-30
- Финансиране от ЕС
- 171 301 €
- Участници
- 1
- Схема
- MC-IEF
Линиите свързват координатора с партньорите.
Накратко на български
Движението и разположението на ДНК в ядрото на клетката се проучват чрез модел с дрожди, като се следят структури като центромерите и теломерите. Разбирането на тези процеси помага при изследването на генетични заболявания, свързани с дефекти в клетъчната обвивка.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Final Report Summary - CHROMATIN MOTILITY (An innovative system to impose altered features on a defined chromosome region and investigate its movement by fluorescence microscopy in the nucleus of a ce
This project aimed at advancing research and training in the life sciences to contribute to Europe's competitiveness and knowledge-based economy. The overall scientific goal was to identify new regulatory mechanisms in DNA compaction and positioning in the cell nucleus. At the training level we successfully acquired expertise in cutting-edge live microscopy imaging and applied the new competencies to further our understanding of nuclear architecture. The project promoted diversification of skills and proactive management of resources, which strengthens the researcher's abilities to lead an independent research team in the near future. The work specifically focused on the nuclear positioning of silent heterochromatin structures that represent vital cellular structures like the centromeres or the telomeres. Their integrity and defined positioning in the nucleus is critical, as defects in these processes are associated with many diseases and genetic disorders such as laminopathies and envelopathies. Therefore the results we obtained have a clear potential relevance to the general public. We created an in vivo system to monitor DNA folding's influence on its organisation in the cell nucleus and vice-versa in the fission yeast model organism. This is both a scientific and technical achievement that advances our understanding of the nuclear regulatory processes. We exploited the system to gain insights into the causality relationship between chromatin structure and position. Surprisingly, we provide evidence that the chromatin state can be modified upon relocalisation and that this phenomenon is dependent on the expression level of the tether. After publication the quality and relevance of the results surely will make a significant contribution to the field. The support from the European Commission provided numerous opportunities for training at workshops and dissemination of the work with posters and seminars at conferences worldwide. Over the course of this project the researcher gained extensive interdisciplinary expertise that will significantly strengthen his professional maturity. Overall the project is a great success as demonstrated with the wide range of contributions made to the research field from scholarly publications to poster presentations and seminars. We also promoted outreach activities through articles in scientific magazines and layperson talks. Our results suggest that the mystery of chromosome compaction and positioning is only beginning to unravel. We will need to persevere in our efforts to provide further insights into the complex relationships between these processes.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Our objective is to provide world-class research and training in the field of Life Sciences. Our innovative project applies interdisciplinary methods to identify new paradigms therefore contributing to the knowledge-based economy and society. Scientifically, the project involves cutting-edge microscopy in fission yeast to characterize the relationship between different chromatin features and their position in the nucleus. We will integrate distinct scientific approaches to obtain breakthroughs in the field. All research activities are in compliance with fundamental ethical principles. Further the project will be carried out in a laboratory where gender equality is encouraged, currently there are ten women in a group of fifteen. With respect to the training: we provide a comprehensive experimental work-plan which is designed to enhance the researchers career development and the diversification of his abilities at an advanced level. This is achieved by sharing reagents and expertise in collaborative exchanges as well as in meetings and workshops. We also present a timetable of the anticipated progress with milestones, promoting proactive project management. The diversity of valuable skills acquired will certainly benefit the researcher and support professional maturity in attaining an independent position. The candidate is a motivated and enthusiastic experienced researcher with an excellent academic and research track record. He developed his skills and scientific training on an international basis, convinced that mobility is central to the open-minded requirement of fundamental discoveries. He will join the well-known laboratory of Professor Robin Allshire in Edinburgh, who is recognized as world-leader in the field of chromosomal biology. Overall the project is a collaborative effort set in the leading research University in Scotland with state-of-the-art infrastructures and facilities, contributing to Europe’s excellence and attractiveness.
Оригинален текст от CORDIS (на английски).
Участници
- THE UNIVERSITY OF EDINBURGH · EdinburghКоординаторОбединеното кралство
Връзки
Данни: CORDIS, © Европейски съюз