DCN · Dynamic Combinatorial Nanoparticles for Protein Surface Recognition
7РП — „Хора“ (Действия „Мария Кюри“)
- Период
- 2010-01-01 → 2011-12-31
- Финансиране от ЕС
- 169 992 €
- Участници
- 1
- Схема
- MC-IIF
Линиите свързват координатора с партньорите.
Накратко на български
Златни наночастици се използват за разпознаване на биомолекули, като например откриване на специфични нишки ДНК. Това помага за разработването на методи за по-точно идентифициране на биологични структури в организма.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Dynamic Combinatorial Nanoparticles for Protein Surface Recognition
We prepared gold nanoparticles (AuNPs) decorated with functional groups as the platform of dynamic combinatorial chemistry (DCC) for biomacromolecule recognition purpose. For bio-compatibility reason, hydrazone chemistry was employed in water at physiological pH in the presence of aniline as catalyst. AuNPs were pre-coated with aldehyde groups, and then further multi-functionalised in dynamic combinatorial libraries (DCLs) by reacting with a pool of hydrazides carrying potential binding sites. To obtain water-soluble gold nanoparticles functionalised with aldehyde, different aldehyde ligands and water-solubilising ligands were synthesised. AuNPs with aldehyde groups were successfully prepared with these ligands by different methods. The size of AuNPs was determined to be proper for biomacromolecule recognition (< 15 nm). The formation and exchange of the hydrazones were confirmed with various characterisation methods by adding hydrazides one after another, and no aggregation or obvious morphology change was observed during this process. After the reversible hydrazone chemistry reached the thermodynamic equilibrium, the hydrazides were cleaved from the surface of AuNPs through hydrazone / oxime exchange and product distribution was analysed. The recognition of biomacromolecules were carried out by exposing the DCL to short sequence double stranded DNAs. The equilibrium of DCL was significantly shifted and cationic hydrazide, which showed strong interaction with polyphosphate backbones of DNA, was amplified by 14 times. Some other bio-interesting molecules such as nucleoside phosphates also showed template effect on the DCLs.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Recent developments in genomics and proteomics have generated an urgent need for new methods of interrogating and targeting biochemical networks. In particular new means of interfering with protein-protein interactions are in high demand and of considerable therapeutic potential. New materials are needed that can recognise and bind to specific protein surfaces. We aim to develop a new approach to the recognition of proteins using individual nanoparticles and dynamic networks of nanoparticles. This requires the development of: 1. Functional nanoparticles capable of selectively recognising proteins. We will explore the potential of dynamic combinatorial chemistry to direct the functionalisation of the surface of the nanoparticles. 2. A means of translating nanoparticle-protein interactions into a signal. Our approach is to use nanoparticle networks that respond to the presence of proteins with a redistribution of their surface functionalisation, which, in turn, produces an optical readout through fluorescence resonance energy transfer. The project is critically dependent on the host's expertise on dynamic combinatorial chemistry and the applicant's expertise on the characterisation of nanostructures. The expected outcome is a conceptually new means of interfacing functional nanoparticles with biomolecules, based on an innovative molecular network approach. This systems chemistry strategy breaks with the reductionist tradition that has characterised most research in chemistry for the last centuries.
Оригинален текст от CORDIS (на английски).
Участници
- RIJKSUNIVERSITEIT GRONINGEN · GroningenКоординаторНидерландия
Връзки
Данни: CORDIS, © Европейски съюз