PAIN_TRPV2 · Single Particle Cryo-Electron Microscopy and Biophysical Characterization of Transient Receptor Potential Vanilloid 2 channel: a Three-Dimensional Structure Characterization of a Membrane Nociceptor
7РП — „Хора“ (Действия „Мария Кюри“)
- Период
- 2009-08-01 → 2012-07-31
- Финансиране от ЕС
- 248 814 €
- Участници
- 1
- Схема
- MC-IOF
Линиите свързват координатора с партньорите.
Накратко на български
Структурата на протеиновия канал TRPV2, който действа като рецептор за болка в мембраните на клетките, се анализира чрез криоелектронна микроскопия. Това помага за разработването на нови лекарства за лечение на заболявания, свързани с работата на тези рецептори.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Single particle cryo-electron microscopy and biophysical characterization ...: A three-dimensional structure characterization of a membrane nociceptor
The training of fellows and transfer of knowledge are essential tools for society. In this now finalised project, Europe has consolidated successfully the training of a future leader in research and the transfer of knowledge towards Europe between two prestigious institutions, such as Harvard University and Autonomous University of Barcelona and the establishment of a mutually beneficial collaboration between the United States (US) and Europe. Excitingly, the international outgoing fellowship (IOF) project has initiated a scientific collaboration between institutions, still ongoing, of extreme importance for the potential scientific and social outcome. Scientifically, the training achieved involves multidisciplinary aspects ranging from neurosciences to structural biology, including high-throughput biotechnology processes and computational biology. Getting into specifics, the project has dealt successfully with breaking barriers for the expression and purification of eukaryote membrane proteins for low-to-high resolution structural biology, indispensable tool for drug design-based approaches leading towards therapeutics of diseases related to human physiological membrane receptors, transporters and channels. Currently, the representation in scientific publications is already high in number and quality and several more manuscripts are in preparation. The results have been disseminated in international and national scientific conferences and workshops and the impact of Marie Curie actions has been explicitly acknowledged in social media. Technological and scientific aspects of the project have been already included within undergraduate teaching lectures and a 'Marie Curie summer school' has been piloted during 2012 and it will be hopefully established for 2013. For the fellow, the project provides a unique opportunity for establishment within the European research community, which has already started in the form of competitive fundraising to continue the research project in Spain. The researcher currently leads a group of four people embedded in the Autonomous University of Barcelona. The establishment of this research group within the European research network will contribute to the excellence and social improvement of Europe.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
The IOF call is a unique opportunity for the researcher to carry out this project. Transient Receptor Potential channels are extremely interesting since some of them are receptors for pain (nociceptors). The actual subject of research is the structural characterization of TRPV2, a nociceptor which responds to different stimuli such as cannabidiol, pressure and noxious heat. TRPV2 is expected to be a large membrane protein (potential homotetramer of ~400 KDa). To study TRPV2’s 3D structure, single particle Cryo-Electron Microscopy is the most suitable method. Further biophysical characterization of the inactive and active states of TRPV2 by FTIR spectroscopy and differential scanning calorimetry will be carried out. The research results will contribute to drug therapeutics on acute and chronic pain treatment. The Outgoing phase will take place at Brigham and Women’s Hospital (affiliated to Harvard Medical School, Boston U.S.A.). The present proposal gathers a skilful and experienced Outgoing host in single particle Cryo-Electron Microscopy (Supervised by Dr. Montserrat Samsó) with a talented and experienced young researcher (Dr. Alex Perálvarez-Marín) in order to generate a transfer of knowledge to a European host. This is the recently created Centre d’Estudis en Biofísica (Supervised by Prof. Esteve Padrós), in the Department of Biochemistry and Molecular Biology at the Universitat Autònoma de Barcelona (Spain), which is looking for a potential enhancement in its Structural Biology section. The Return host is embedded in Barcelona’s unique scientific environment. The ongoing Centre d’Estudis en Biofísica and the Mare Nostrum Supercomputing facilities, in addition to the new Alba Synchrotron and the planned Institute for Structural Biology, are a very suitable environment to reintegrate the researcher by means of this advanced and innovative research project, expected to strongly contribute to the European excellence and competitiveness.
Оригинален текст от CORDIS (на английски).
Участници
- UNIVERSITAT AUTONOMA DE BARCELONA · Cerdanyola Del VallesКоординаторИспания
Връзки
Данни: CORDIS, © Европейски съюз