PPOEEC · Physical Properties, Organization and Evolution of Eukaryotic Chromosomes
7РП — „Хора“ (Действия „Мария Кюри“)
- Период
- 2008-10-01 → 2012-09-30
- Финансиране от ЕС
- 100 000 €
- Участници
- 1
- Схема
- MC-IRG
Линиите свързват координатора с партньорите.
Накратко на български
Структурата и организацията на еукариотните хромозоми се анализират чрез методи за проследяване на хроматина при дрожди. Това помага за разбирането на процеси като ремонта на ДНК и поддържането на стабилността на генома.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Periodic Report Summary - PPOEEC (Physical Properties, Organization and Evolution of Eukaryotic Chromosomes)
The present report covers the period from October 2010 until February 2011, because of the early termination of the IRG contract. Indeed, Dr. Koszul applied successfully to run an independent laboratory in the Institut Pasteur during the year 2010. He was also granted with an ERC starting grant. Given the negotiation procedure in the framework of an ERC Starting Grant, it appeared it was convenient to terminate the IRG funding, hence the short reporting period 2. During these few months, Dr. Romain Koszul kept on working on the projects detailed in the first period report. The projects aimed at a better understanding of the structure, dynamics and organisation of eukaryote chromosomes, and their influence on DNA-related metabolic processes (DNA repair, transcription, etc.) This proposal aimed at using interdisciplinary approaches to characterise some of the links that bound eukaryote chromosomes organisation with biological processes, with an emphasis on the maintenance of genome stability. We aimed at 1) developing a genomic chromosome conformation capture (3C) assay in order to decipher chromatin organisation of yeast chromosome during cell-cycle and 2) designing a microfluidic system that would help us to micromanipulate isolated cells and nuclei in order to determine the cellular response to a mechanical signal. During this reporting period, we pursued our effort to develop genomic 3C methods and analysis tools, one of the primary objectives from the proposal. In addition, thanks to the IRG funding Dr. Koszul attended and presented some of his results at a meeting organised by the Biophysical Society (USA) on the interplay between cytoskeleton, the nucleus and the chromatin, which is also directly connected to his research interest. He also worked on a historical perspective on an key study in the history of genetics, which is now published in the journal Genetics.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
The remarkable structure and the key role hold by the DNA molecule in the living world raise numbers of fascinating questions with regard to its apparition and its evolution. However, little is known so far about the potential interplay between the physical status of chromosomes and their diverse biological functions. I aim at addressing these questions. As a postdoc I work at the interface of several disciplines, investigating physical properties of eukaryotic chromosomes as well as aspects of nuclear dynamic. In this regard, we recently proposed that variations in the chromatin compaction status could account for different types of chromosomal dynamics observed during meiotic prophase. In parallel to these studies, one of the primary goals of my research has been to achieve virtual trapping and manipulation of chromatin or individual chromosomes in a controlled environment. My objective is now to pursue the development of functional, user-friendly, devices in the host organization and develop long-term collaborations across EU member states with scientists from various disciplines. In addition to this in vitro approach, I propose to investigate further the organization of chromosomes/chromatin during replication, and their impact onto genome structure. Using the 3C/5C technology partially developed in my current laboratory, I will perform a genome – wide mapping of yeast replication factories to determine whether large intra or inter-chromosomal regions are coordinately replicated in the same replication factories or not. Furthermore, I propose to investigate whether some DNA regions involved in specific chromosomal rearrangements interact preferentially because of transient co-localization during replication within a same factory. This will provide a new understanding of the “contact-first” model for chromosome rearrangement and deepen our insight onto chromosome evolution, and cancer.
Оригинален текст от CORDIS (на английски).
Участници
- CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS · ParisКоординаторФранция
Връзки
Данни: CORDIS, © Европейски съюз