ROLE OF SPIS · The role of Salmonella Pathogenicity Islands in salmonellosis outbreaks and invasive disease
7РП — „Хора“ (Действия „Мария Кюри“)
- Период
- 2010-02-01 → 2014-01-31
- Финансиране от ЕС
- 100 000 €
- Участници
- 1
- Схема
- MC-IRG
Линиите свързват координатора с партньорите.
Накратко на български
Генетичните острови на патогенност при бактерията Salmonella определят дали тя ще причини обикновен стомашен грип или по-тежка инфекция в кръвта. Разбирането на тези механизми помага да се разбере как бактериите еволюират, разпространяват се през хранителните вериги и развиват устойчивост към антибиотици.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
The role of Salmonella Pathogenicity Islands in salmonellosis outbreaks and invasive disease
Salmonella enterica is a facultative intracellular human and animal bacterial pathogen responsible for global pandemics of foodborne infections, posing a major threat to public health. This highly versatile pathogen can infect a broad range of hosts and causes different clinical outcomes. The single species S. enterica includes more than 2,600 serovars that share high sequence similarity and are taxonomically classified into six subspecies. Subspecies I serovars, adapted for mammals and avian hosts, are associated with more than 99% of all Salmonella infections in humans. The majority of non-typhoid Salmonella (NTS) infections in humans presents as gastroenteritis, however, about 5% may be invasive, and manifest as bacteremia or other extra-intestinal infections. Many of the NTS serovars are capable of colonizing the intestines of livestock with potential risk of contaminating the food chain and therefore, salmonellosis is often associated with animal products and produce. Many of the Salmonella virulence factors are encoded on mobile elements with variable presence between different serovars and even across isolates within the same serovar. Genomic diversity between related bacteria is largely determined by gain of functions via horizontal gene transfer (HGT). Mobile and integrative genetic elements, including plasmids, bacteriophages, transposons, integrons, integrative and conjugative elements (ICEs), and pathogenicity islands (PAIs) are very important vehicles of HGT enabling transmission of genetic information between bacteria. Horizontally acquired foreign DNA elements often encode genes that can profoundly affect the fitness, antimicrobial resistance (AMR) and the lifestyle of their bacterial host and therefore play a key role in bacterial evolution. Different projects in my lab are aimed to better understand on the molecular level, how differences between Salmonella serovars, or genetic variation between strains of a specific serovar affect Salmonella pathogenicity and host-pathogen interactions. Thanks to the generous support of the Marie Curie Reintegration Grant we were able to study the following projects: 1. Genetic and phenotypic variation among non-typhoidal Salmonella serovars accounted for invasive disease in humans. 2. Integrative analysis of Salmonella outbreaks and invasive salmonellosis in Israel. 3. The epidemiology and molecular evolution of S. Infantis emergence in Israel. 4. Molecular and Cellular Characterization of a Salmonella enterica Serovar Paratyphi A Outbreak Strain and the Human Immune Response to Infection. 5. The role of the Salmonella enterica PhoP in regulation of Horizontally Acquired genes. During this time, the results of our research were presented in multiple national and international scientific conferences and published as five peer-reviewed papers in leading journals. Additional paper will be submitted in the very near future.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Non-typhoid Salmonella (NTS) enterica is a major cause of foodborne infections in industrialized countries and a leading cause of hospitalization and death among bacterial foodborne pathogens. The vast majority of the Salmonella virulence factors are encoded within horizontally acquired discrete regions along the chromosome known as Salmonella Pathogenicity Islands (SPIs). SPIs are crucial for Salmonella virulence. Herein we propose to study the role of 17 SPIs and associated virulence loci in different aspects of NTS pathogenicity including the ability to cause outbreaks, host-to-host transmission, and manifestation of invasive disease. In the first stage of the study we will map and characterize the distribution of these virulence loci across various outbreak and bacteremia isolates. This analysis will provide a broad view over the distribution of SPIs in highly virulence isolates, which are clinically relevant in Israel and Europe. Based the collected data, we will develop a Quantitative Microbial Risk Assessment approach for detection of virulent Salmonella strains in food, environmental and livestock samples. In addition we are interested in investigating the potential contribution of SPIs to the oral-fecal route of transmission. To do so, we will use the persistent mouse model and study the ability of Salmonella strains, harboring SPIs deletions to transmit the disease over time. The gained data are expected to yield a broader understanding about the role of primary virulence loci in the pathogenesis and epidemiology of Salmonella and will shed light on the evolution of pathogenic Salmonella serotypes. Understanding these mechanisms will contribute to our knowledge about host-pathogen interactions and the molecular basis of outbreaks. Deeper understanding about these processes could lead to better prevention and treatment of infectious diseases and may provide new therapeutic targets for the development of novel antimicrobial chemotherapies and vaccines.
Оригинален текст от CORDIS (на английски).
Участници
- MEDICAL RESEARCH INFRASTRUCTURE DEVELOPMENT AND HEALTH SERVICES FUND BY THE SHEBA MEDICAL CENTER · Ramat GanКоординаторИзраел
Връзки
Данни: CORDIS, © Европейски съюз