SUSY · Protein SUlfenylation in Medicago truncatula – Sinorhizobium meliloti SYmbiosis
7РП — „Хора“ (Действия „Мария Кюри“)
- Период
- 2009-10-31 → 2011-10-30
- Финансиране от ЕС
- 30 000 €
- Участници
- 1
- Схема
- MC-ERG
Линиите свързват координатора с партньорите.
Накратко на български
Протеините при взаимодействието между растението Medicago truncatula и бактериите Sinorhizobium meliloti се анализират за промени, предизвикани от водородния пероксид. Това помага да се разбере как този химичен сигнал влияе върху образуването на коренови възелчета, които фиксират азота от въздуха.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Protein sulfenylation in Medicago truncatula - Sinorhizobium meliloti symbiosis
There is now compelling evidence that reactive oxygen species (ROS) and in particular hydrogen peroxide (H2O2), is an important signal in plants. This has been particularly reported in the case of plant - pathogen interactions. More recently, the involvement of ROS during the Legume - Rhizobium symbiotic interaction has also been highlighted; H2O2 appears to be involved in the development and functioning of this symbiosis (Pauly et al. 2006; Marino et al., 2011), which is characterised by the formation of a new organ on the root, the so-called nodule, able to fix atmospheric nitrogen. On the other hand, H2O2 is known to interact directly with protein thiols, leading to the formation of a sulfenic acid group (sulfenylation); this has been suggested as a widespread mechanism by which H2O2 might affect protein function (Cooper et al., 2002). In this framework, the aim of the SUSY project, which was launched on 1 November 2009 and ran for two years, was to identify sulfenylated proteins in the Medicago truncatula - Sinorhizobium meliloti symbiotic interaction. Indeed, this was crucial to improve our knowledge on the role of H2O2 in this symbiosis. This project was clearly based on a multidisciplinary approach (proteomic, physiology, biochemistry, molecular and cell biology and bioinformatics) and aimed at characterising a completely new role for H2O2 as a signalling molecule in plants, in providing a repertoire of proteins regulated by H2O2-driven post-translational modifications. The main other goal of the project was to define the impact of the cysteine sufenylation of protein of interest at a physiological level, to understand the role of this enzyme in the symbiosis.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
It now appears that plants purposefully generate Reactive Oxygen Species (ROS) such as Hydrogen Peroxide (H2O2) as signalling molecules to control various processes. There is now compelling evidence that H2O2 is an important signal in plants; this has been particularly reported in the case of plant – pathogen interactions. Symbiosis between Legumes and Rhizobia is characterized by the formation of a new organ on the root able to fix atmospheric nitrogen. More recently, the involvement of ROS during the symbiotic interaction has also been highlighted where H2O2 production must contribute to the control of the establishment and/or functioning of the symbiosis, by exerting a positive effect on both partners. On the other hand, H2O2 is known to interact directly with protein thiols, leading to the formation of a sulfenic acid group (sulfenylation) which has been suggested as a widespread mechanism by which H2O2 might affect protein function. Thus, the goal of the project is to identify the model plant Medicago truncatula H2O2 targets proteins during its interaction with Sinorhizobium meliloti. The three objectives are: i) isolate specifically the sulfenylated proteins using an affinity purification followed by their proteomic identification, ii) map the H2O2 modified cysteins and validate their role in protein fonction and iii) characterise the role of the sulfenylation during the symbiotic interaction through a functional in planta analysis. The project aims at characterizing a completely new role for H2O2 as a signalling molecule in plants. The research programme will lead to the identification and the characterisation of H2O2-driven post-translational signalling. This will lead to important breakthroughs not only in the symbiotic field but also in compatible plant pathogen interaction and more generally of plant development. In this respect, it is expected to open new fields of investigation.
Оригинален текст от CORDIS (на английски).
Участници
- INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT · ParisКоординаторФранция
Връзки
Данни: CORDIS, © Европейски съюз