REKSARD · Receptor-like Kinase Signalling in Arabidopsis thaliana Root Development
7РП — „Хора“ (Действия „Мария Кюри“)
- Период
- 2011-06-25 → 2014-06-24
- Финансиране от ЕС
- 45 000 €
- Участници
- 2
- Схема
- MC-ERG
Линиите свързват координатора с партньорите.
Накратко на български
Рецепторната киназа ACR4 при растението Arabidopsis thaliana регулира деленето на клетките при развитието на корените. Разбирането на този механизъм помага да се разбере как растенията оптимизират усвояването на вода и хранителни вещества за по-добър растеж и реколта.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Receptor-like Kinase Signalling in Arabidopsis thaliana Root Development
Optimized root architecture is crucial for efficient water and nutrient uptake, and strongly affects plant growth and seed yield. This project focused on the involvement of membrane-associated receptor-like kinases in registering and conveying (positional) information during plant (lateral) root development.The fellow identified the membrane-associated receptor-like kinase ARABIDOPSIS CRINKLY4 (ACR4) - which has been studied during the development of epidermis in maize and Arabidopsis and aleuron in maize (Becraft et al., 1996, Science 273:1406-1409; Gifford et al., 2003, Development 130:4249-4258; Watanabe et al., 2004, Plant J 39:298-308) - as a key regulator of formative cell divisions in the root (De Smet et al., 2008, Science 322:594-597). In this ERG project, the fellow further characterized this homeostatic ligand/receptor signalling mechanism that can integrate mobile signalling molecules to control formative cell divisions during organogenesis and, as such, provided an excellent tool to study short range cell-to-cell communication during growth and development. This was a starting point for the enormous future challenge to figure out which receptor(s) interact(s) with which ligand(s) to generate specific responses in plant and, more specifically, in root development. The research focused on how the receptor-like kinase ACR4 can achieve molecular and developmental flexibility in Arabidopsis thaliana (lateral) root development and had three overall aims divided over three work packages (WP). WP1 and WP2 centred around the downstream events, namely (indirect) transcriptional changes (WP1) and direct substrates (WP2); WP3 focused on the upstream regulation through interaction with (possibly multiple) ligands. To accomplish these goals, we used various interdisciplinary approaches. So far, we have identified and characterized potential ACR4 ligands, but have not (yet) been able to demonstrate a physical interaction between ACR4 and a putative ligand. In addition, we have described transcriptional changes downstream of ACR4 and identified ACR4-interacting proteins and substrates.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
The past decade researchers have mainly focused on the analyses of phytohormone gradients and mobile transcription factors to explain patterning. In Arabidopsis, model processes like primary and lateral root development are well understood on the level of transcriptional changes and hormonal control, but very little attention has been given to an alternative way of cell-cell communication using ligand-receptor-like kinase systems (De Smet et al., 2009, Nat Cell Biol, in press). Lately a number of receptor-like kinases and potential ligands have been described in root development (De Smet et al., 2008, Science 322:494-497; Müller et al., 2008, Plant Cell 20:934-946; Stahl et al., 2009, Curr Biol 19:909-14), making it a perfect time to start investigating how these receptor-like kinases and their respective ligands control the different developmental processes and what their targets are. More and more tools become available to study difficult membrane proteins on the level of 3D structure, phosphoproteomics, and in silico ligand identification. In brief, I will use different and interdisciplinary strategies, based on state of the art transcriptomics, proteomics, interactomics, associomics and more in depth analysis on the single gene/protein/ligand level to describe ligand-receptor-mediated cell-cell communication during root development, and, more specifically, to identify key components in ACR4-dependent signalling. The aspects this project plans to address would contribute extensively to the knowledge of membrane associated receptors-like kinases in general, have rarely been covered focusing on one receptor-like kinase, and will allow elucidating the mechanisms and structural aspects behind this crucial ACF4-dependent signaling pathway, such as: (1) identification of downstream genome-wide transcriptional changes, (2) identification of substrates on a proteome-wide scale, and (3) identification and physical interaction of ligands with the receptor domain.
Оригинален текст от CORDIS (на английски).
Участници
- THE UNIVERSITY OF NOTTINGHAM · NottinghamКоординаторОбединеното кралство
- VIB VZW · ZWIJNAARDE - GENTБелгия
Връзки
Данни: CORDIS, © Европейски съюз