C4TRAN · C4 photosynthesis transcriptomics
7РП — „Хора“ (Действия „Мария Кюри“)
- Период
- 2010-09-01 → 2012-08-31
- Финансиране от ЕС
- 179 603 €
- Участници
- 1
- Схема
- MC-IEF
Линиите свързват координатора с партньорите.
Накратко на български
Генната експресия при растения с C3 и C4 фотосинтеза разкрива кои гени управляват работата на различните клетки в листата. Това помага да се разбере как е еволюционирал сложният механизъм на фотосинтезата при различни видове растения.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Periodic Report Summary - C4TRAN (C4 photosynthesis transcriptomics)
The objectives were to sample leaves of C3 and C4 congeneric pairs for gene expression and to identify patterns of gene expression associated with mesophyll and bundle sheath cells in C4 plants. This was done after developing a bioinformatic pipeline to allow this to be done after ribonucleic acid sequencing (RNAseq), but without genome sequence. We also implemented a new pipeline to allow unambiguous identification of homologous transcripts in different species. This has allowed to map alterations in gene expression as C4 is induced. We have defined genes that were previously not known to be associated with C4 photosynthesis, and also identified transcription factors that underlie these behaviours in gene expression. These transfactors are expressed in either mesophyll of bundle sheath cells. Furthermore, we find that the transcription factor homologues have been recruited in separate lineages of C4 plant indicating that parallel evolution of transfactors has generated this complex trait.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
C4 photosynthesis concentrates CO2 around RuBisCO and so increases the efficiency of photosynthesis. In the vast majority of C4 species the biochemistry of photosynthesis, and also the cell biology and development of leaves is altered. Despite these complex changes, the C4 pathway has evolved at least 45 times independently. Because C4 photosynthesis is more efficient in C4 plants, it has been proposed that engineering the C4 pathway into C3 crops such as rice would be beneficial for productivity. For this to be feasible, we need a better understanding of the basic mechanisms underlying C4 photosynthesis. I aim to investigate mechanisms generating cell specific gene expression in C4 plants, and will use C. gynandra, the most closely related C4 species to the C3 model Arabidopsis. I will generate comprehensive profiles of transcript abundance in mesophyll (M) and bundle sheath (BS) cells using laser capture microdissection and Illumina sequencing. I will also generate knock-down lines of C. gynandra for 20 transcription factors that we have identified as candidates for maintaining patterns of gene expression required for C4 photosynthesis. The project will significantly increase our understanding of basic mechanisms underlying C4 photosynthesis, and therefore may facilitate introduction of C4 traits in C3 crops.
Оригинален текст от CORDIS (на английски).
Участници
- THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE · CAMBRIDGEКоординаторОбединеното кралство
Връзки
Данни: CORDIS, © Европейски съюз