FERFLU EXPRESS · Investigation of influenza immune responses and vaccine efficacy correlates by global expression profiling and immunological analyses in the ferret model of influenza
7РП — „Хора“ (Действия „Мария Кюри“)
- Период
- 2010-09-01 → 2012-08-31
- Финансиране от ЕС
- 239 693 €
- Участници
- 1
- Схема
- MC-IIF
Линиите свързват координатора с партньорите.
Накратко на български
Имунните реакции при грип се анализират чрез промени в гените на заразени куниче. Това помага за създаването на диагностични инструменти за ранно разпознаване на опасни щамове на вируса.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Investigation of influenza immune responses and vaccine efficacy correlates by global expression profiling and immunological analyses in the ferret model of influenza
In this project, we proposed to elucidate the mechanisms involved in host response to influenza infection and determine the key immune responses triggered by different influenza viruses. We suggested a comprehensive analysis of gene expression changes in influenza infected ferrets (Objective 1). These responses were then be compared to the key transcriptional changes with regard to influenza challenge after vaccination in the ferret model (Objective 2) to identify key genetic biomarkers correlating with vaccine protection. We utilized de-novo transcriptome sequencing of a normalized cDNA library from influenza infected ferrets. This allowed us to identify more than 19000 partial ferret transcripts, including more than 1000 gene orthologs known to be involved in the innate and the adaptive immune response. This study shows that utilizing next generation sequencing of a cDNA library is a fast and effective way to generate molecular analysis tools for non-standard animal models. We also analyzed the gene expression changes induced by different closely related influenza strains in the ferret model. The analysis revealed a common set of genes being either up or down regulated by all three strains investigated. However, the bulk of the identified expression changes showed strain specific patterns. Using sophisticated statistical algorithms, we were able to isolate a small number of genes that could predict both the infectious strain and the lung pathology in a set of unknown samples. The results show promise for further development of diagnostic tools, based on gene expression profiles of genetic biomarkers in blood. Such diagnostic tools could be used for early identification of patients infected with highly pathogenic strains, which could prevent transmission of more virulent virus strains, and more importantly, increasing the possibility of starting early treatment in these patients will spare both life and health care resources.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Pandemic influenza, such as the 2009 H1N1 influenza virus strains that recently emerged in Mexico and in the US, can have serious effects on human health. Development of vaccines that are able to elicit more potent and protective immune responses as well as reducing the impact of future pandemic outbreaks is critical. Successful development of such vaccines is dependent on animal models to test vaccine efficacy and safety. Animal modeling of infectious disease also allows for transcription mapping of the cellular and molecular mechanisms by which these viruses infect as well as how the vaccines against them provide protection. We will use the ferret influenza model to build a comprehensive map the genes signatures involved at the different stages of the immune response to influenza virus infection. We will also analyze the transcriptional profiles induced by vaccination using standard inactivated and live attenuated influenza vaccine currently being administered to humans. In addition, we will study the gene expression responses triggered by virus challenge in vaccinated animals. Careful mapping of the gene responses triggered by influenza infection, as well as the mechanisms the virus use to overcome these responses will provide key information for development of vaccines and antiviral drugs against epidemic and pandemic influenza strains.
Оригинален текст от CORDIS (на английски).
Участници
- KAROLINSKA INSTITUTET · STOCKHOLMКоординаторШвеция
Връзки
Данни: CORDIS, © Европейски съюз