IronAlgae · Iron uptake in Chromera velia and other marine microalgae
7РП — „Хора“ (Действия „Мария Кюри“)
- Период
- 2011-04-01 → 2014-03-31
- Финансиране от ЕС
- 45 000 €
- Участници
- 1
- Схема
- MC-ERG
Линиите свързват координатора с партньорите.
Накратко на български
Механизмите за усвояване на желязо при морски микровоалги, като Chromera velia и диатомеите, се анализират чрез сравнение на различни източници на този метал. Това помага да се разбере как микроорганизмите оцеляват в океанска среда с много ниски нива на достъпно желязо.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Iron uptake in Chromera velia and other marine microalgae
Marine microorganisms must have evolved strategies to adapt to the extremely low bioavailability of iron. However, the current understanding of the mechanisms of iron metabolism in phytoplankton remains elusive. Recently, a novel nonreductive two-step model of iron uptake was described in cells of the marine alga Chromera velia. In this project, we have compared iron requirement and iron uptake mechanism proposed for C. velia with common micro-algae belonging to different groups: Ostreococcus tauri and Micromonas pusilla (Chlorophyta), Emiliania huxleyi (Coccolithophores), Phaeodactylum tricornutum and Thalassiosira pseudonana (Diatoms). We have shown that iron binding at the cell surface is a critical step of iron uptake mechanisms in marine micro-algae and that most microalgae can take up both ferric and ferrous iron, regardless of the presence of a ferrireductase system. Using proteomic approach we identified candidate proteins involved in iron uptake/storage in O. tauri and P. tricornutum, respectively. Furthermore, we compared ferric EDTA, ferric citrate and ferrous ascorbate as iron sources to study iron metabolism in micro-algae. While ferric EDTA is a better iron source than ferric citrate for growth and chlorophyll levels, the use of ferric citrate and ferrous ascorbate enables the molecular components that rapidly bind iron in cells of marine micro-algae to be identified.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Recently discovered marine alga Chromera velia represents the closest known free living photosynthetic relative to obligate parasites Apicomplexa. C. velia is easy to cultivate, thus representing a powerful model for studying organisms living in nutritionally poor ocean environment as well as a model to study the evolution of parasitism. For most marine microorganisms the mechanisms of adaptation to extremely low iron levels in surface seawater are unknown. We have recently described in C. velia a novel non-reductive two-step model of iron uptake. In this project we will further study the mechanism of iron uptake system in C. velia and characterize it at the molecular level. Furthermore, we will compare iron requirement, uptake mechanism and storage in various microalgae belonging to different phyla. Our preliminary experiments suggest great diversity in the requirement for iron among different marine microalgae. We will try to elucidate if these differences are related to the diversity of the mechanisms of iron acquisition and storage.
Оригинален текст от CORDIS (на английски).
Участници
- UNIVERZITA KARLOVA · Praha 1КоординаторЧехия
Връзки
Данни: CORDIS, © Европейски съюз